ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (5): 1057-1061.doi: 10.11843/j.issn.0366-6964.2016.05.025

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Effect of Vitamin C on Tibetan Boar Semen Preserved at 4 ℃ and Levels of the Sperm Global Genome DNA Methylation

REN Zi-li,ZHAO Yan-ling,WANG Jian-zhou,LI Yu-xin,SHANG Peng,CHAMBA Yangzom*   

  1. (Faculty of Animal Science,Tibet Agricultural and Animal Husbandry College,Linzhi 860000,China)
  • Received:2015-11-25 Online:2016-05-23 Published:2016-05-23

Abstract:

This research was conducted to study the effect of vitamin C on Tibetan boar semen preserved at 4 ℃ and the subsequent influence on global genome DNA methylation.The semen was collected from Tibetan boar and preserved at 4 ℃ in modified medium supplemented with vitamin C at 0.0,2.5,5.0,7.5,10.0 g•L-1.Sperm motility,abnormality,acrosome integrity and plasma membrane integrity were evaluated 5 days after preservation.And the global methylation level of the fresh and preserved sperm was detected by using MethylFlashTM.The results showed that a significantly higher sperm motility and plasma membrane integrity,acrosome integrity and less abnormality was observed in group supplemented with 5.0 g•L-1 vitamin C(P<0.05).DNA methylation levels of fresh sperm (0.604 7±0.040 3) ng and sperm preserved with vitamin C (0.656 9±0.048 3) ng were significantly lower than those preserved without vitamin C (0.920 2±0.016 9) ng (P<0.05),but no significant difference was found between fresh semen and sperm preserved with vitamin C(P>0.05).These results indicated that supplementation of vitamin C at 5.0 g•L-1 semen extender could improve quality of Tibetan boar semen preservation at 4 ℃ and mitigate effect on DNA methylation of Tibetan boar sperm.The results in the current study would be helpful for further investigation of the freezing protocol for Tibetan boar semen.

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